7/24/2019 · Agilent InstantPC (formerly ProZyme) labeled glycan standards and libraries are commonly used as qualitative standards for LC/FLD and LC/MS. These standards come pre-labeled with InstantPC, ready to reconstitute and inject. Better detection of low abundance N-glycans in your LC/FLD or LC/MS method translate to higher method sensitivity.
? Agilent AdvanceBio Gly-X N-Glycan Prep with InstantPC Kit, 96-ct User Manual 13 5 Add 100 µL of Gly-X InstantPC Eluent to each well. Using vacuum, (2 or less inHg) elute samples into the Collection Plate. 6 Release the vacuum, remove the Cleanup Plate, and rest on the Storage Plate. 7.
Agilent AdvanceBio Gly-X with InstantPC and Gly-X 2-AB Express kits (formerly ProZyme) for the release of N-glycans using PNGase F followed by instant glycosylamine labeling with InstantPC or reductive amination labeling with 2-AB Express, respectively. Labeled N.
Glycan ProZyme name Oxford name1 CFG structure Unlabeled 2 lnstantPC lnstantAB 2-AB 2-AA lnstantQ APTS G3 NA3 A3G(4)3 GKC-035300 GKSB-308 GKSA-308 G3S3 ?(2,6) A3(a2,6) A3G(4)3S(6)3 GKC-335300 GKSB-314 GKSQ-314 A4 NGA4 A4 GKC-006300 GKSB-309 GKSA-309, InstantPC ™ dye is a novel instant glycan labeling reagent for MS that provides markedly increased MS and fluorescent sensitivity. InstantPC forms a stable urea linkage with glycosylamines released by rapid N-Glycanase (PNGase F) digestion.
12 Agilent Instant Pilot Users’s Guide n o i t a m r o f n I p u – t r a t 1S Instant Pilot Features Instant Pilot Features The Agilent Instant Pilot (IP) provides complete local control and monitoring of a single module or an entire Agilent 1100/1200/1260/1290 Series HPLC system. You have easy access to every supported function,
